In bacteria, the best conserved promoter feature is the AT-rich element; a sequence essential for DNA unwinding. Further elements, and gene regulatory proteins, are needed to recruit RNA polymerase to the sequence. Hence, elements cannot function in isolation. Consequently, sequences that resemble the element occur frequently. However, it is not clear how RNA polymerase initially recognizes such sequences. Here, we identify a non-canonical promoter element that plays a key role.
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